Monoclonal antibody 3/11 recognizes C3a, the activating fragment of complement protein C3. C3a is a 190 kDa protein and the highest content of complement protein in serum. C3 is the third part of the complement system and plays a central role in complement activation. The three pathways converge at this key molecule. The cleavage of the key complement component C3 leads to the production of a series of effector molecules with multiple biological functions. The cleavage product C3b promotes the cultivation and subsequent formation of complement. C3a is a small Molecular lysate (approximately 10 kDa), with allergic toxicity and immunomodulatory properties, works through a specific receptor (C3aR), which belongs to the family of G protein-coupled receptors. C3aR- is expressed in a variety of cells, Including bone marrow and non-bone marrow cells, are expressed on hematopoietic stem/progenitor cells. It has been shown that S can promote the transport/homing of these cells to BM. The participation of C3aR on DC and T cells has been shown to upregulate these cells Function.
Monoclonal antibody 3/11 reacts specifically with C3a anaphylactoxin and provides a tool for monitoring acute complement activation (C3a production) in experimental mouse models.