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Amerigo Scientific

ROS Detection Assay Kit -for Transfection Assay | Amerigo Scientific

TheROS Detection Assay kit (Green Fluorescence)is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2’, 7’-Dichlorodihydrofluorescein diacetate (DCF-DA).

Once DCF-DA has diffused into cells it is deacetylated by cellular esterases to a non-fluorescent compound and rapidly oxidized by ROS into DCF. DCF is highly fluorescent and can be detected by microscopy, titration in microplate and also cytometry (ex 485/em 535). The fluorescence intensity that results is proportional to the ROS levels within the cell./

Main Features

Measure ROS production in live cells

Ideal for fluorescence microscopy, microplate titration and cytometry

Compatible with adherent cells as well as suspension cells

Kit content

For up to 300 assays in 96-well plate

25mM DFC-DA in DMF (35µL) -10X ROS Buffer (5 mL)

Tert-Butyl Hydrogen Peroxide (TBHP) 25 mM (50 µL)

Each ROS Kit provides sufficient reagents for approximately 300 measurements in microplate format and includes Tert-Butyl hydroperoxide (organic peroxide) solution provided as a positive control for ROS generation.

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ABOUT THE COMPANY

Amerigo Scientific is a distribution company focused on providing critical products and services to the biomedical and life science research communities. Although a new company, Amerigo Scientific's founder has had more than 20 years of rich experience in the biomedical and biochemical fields, and has established close contacts with key personnel in...
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