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Amerigo Scientific

ONPG Kit -for Transfection Assay | Amerigo Scientific

ONPG assay kit is ideal to quantify high expression level of beta-Gal. The levels of active beta-Gal expression can be quickly measured by its catalytic hydrolysis of ONPG (o-nitrophenyl-beta-D-galactopyranoside) substrate to a bright yellow product (Absorbance at 405-420nm).LacZ is one of the most frequently reporter gene used in transfection experiments. The LacZ encoded protein (beta-galactosidase, beta-Gal), is very stable, resistant to proteolytic degradation and easily assayed.

All the necessary reagents are provided in this assay kit and offer a rapid, simple and sensitive method to quantify spectrophotometrically the enzyme expression level in LacZ transfected cells.

Convenient for all transfection assays

Suitable for cultured cells and tissues

Simple, rapid and easy-to-use

Economical

Optimized with stable buffers

Kit contains 5X Lysis Buffer 55 mL, Standard Dilution Buffer 55 mL, ONPG Substrate Solution 55 mL, Stop Buffer 55 mL, Beta Gal Enzyme Standard 100 µL

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ABOUT THE COMPANY

Amerigo Scientific is a distribution company focused on providing critical products and services to the biomedical and life science research communities. Although a new company, Amerigo Scientific's founder has had more than 20 years of rich experience in the biomedical and biochemical fields, and has established close contacts with key personnel in...
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