Name: CPRG Kit -for Transfection Assay
Availability: InStock

CPRG Kit -for Transfection Assay | Amerigo Scientific

CPRG assay kit is ideal to quantify low expression level of beta-Galactosidase. LacZ is one of the most frequently reporter gene used in transfection experiments. The LacZ encoded protein (beta-galactosidase, b-Gal), is very stable, resistant to proteolytic degradation and easily assayed.

The levels of active beta-Gal actosidase expression can be quickly measured by its catalytic hydrolysis of CPRG (Chlorophenol red-b-D-galactopyranoside), substrate to a dark red product. The high sensitivity of this substrate improves the measurement of beta-Galactosidase activity when the reporter gene expression is low. (Absorbance at 570-595 nm).

All the necessary reagents are provided in this assay kit and offer a rapid, simple and sensitive method to quantify spectrophotometrically the enzyme expression level in LacZ transfected cells.

Convenient for all transfection assays

Suitable for cultured cells and tissues

Simple, rapid and easy-to-use

Economical

Optimized with stable buffers

Kit contains: Lysis Buffer 55 mL, Standard Dillution Buffer 55 mL, 10X CPRG 5 mL, Substrate Buffer 55 mL, Stop Buffer 55 mL, Beta Gal Enzyme 100 µL

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