Partially purified tRNALys contains tRNALys1,2, tRNALys3 and other unknown tRNAs. The partial purified tRNALys is extracted from human placenta with the following procedures: (1) the extraction of total RNA by phenol-chloroform; (2) the chromatogram extraction of total tRNA with DE52; and (3) the chromatogram separation of total tRNALys from total tRNA with DEAE- Sephadex (A50). Aminoacylation of tRNA with H3-lableled lysine using aminoacyl tRNA synthetase from bovine liver is used for detection.This product can be used to initiate reverse transcription or to carry out hybridization in a specific, but an unquantitative manner. 
Source: human placenta 
Purity: DEAE grade.