Diglycyl-lysine antibody (clone GX41) recognizes lysine residues modified by diglycine, an adduct left at sites of ubiquitination after trypsin digestion. Diglycyl-lysine antibody is produced by immunizing animals with a synthetic diglycine-modified histone peptide and established in hybridoma line GX41. This monoclonal antibody is purified by protein A affinity chromatography./

Data/



Figure 1: GX41 antibody recognizes the diglycyl-lysine moiety at ubiquitinated sites after trypsin digestion./



Figure 2: Specificity of anti-diglycyl-lysine (clone GX41) is verified by western blot analysis of beta-lactoglobulin, lysozyme, or rat brain lysate, in which the lysines were either unmodified (A), or modified with Boc-Gly-Gly (B) or Gly-Gly (C) adducts, respectively. As shown above, the GX41 exhibits no detectable reactivity with proteins unless the lysines are modified with Gly-Gly./



Figure 3: Equimolar amounts of the two peptides (GGDRVYIHPFHL and Ac-SYSMEHFRWGK*PV-NH2) were immunoprecipitated with immobilized GX41 antibody and analyzed by MALDI-TOF MS. K* refers to a lysine with a Gly-Gly modification. As shown above, the unmodified peptide is absent from the immunoprecipitate, while the peptide containing the Gly-Gly modification is immunoprecipitated.